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KMID : 0900919960200010001
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Korean journal of Animal Reproduction
1996 Volume.20 No. 1 p.1 ~ p.8
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Addition of Oviductal Fluid to the Fertilization Medium Enhances Monospermic Penetration and Subsequent In Vitro Development of Porcine Oocytes
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Moon Seung-Ju
Lim Joon-Gyo
Lee Hoon-Taek
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Abstract
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The objective of this study was to determine effects of oviductal fluid on the sperm penetration and subsequent in vitro development of porcine oocytes. The addition of oviductal fluid to the fertilization medium decreased sperm pen etration and the mean number of spermatozoa in penetrated eggs. The number of spermatozoa firmly bound to zona pellucida was also decreased in the presence of oviductal fluid. Chlortetracy cline (CTC) fluorescence patterns were used to determine incidence of capacitation and acrosome reaction. The proportion of capacitated a and acrosome free spermatozoa increased when spermatozoa were exposed for 1.5 and 3 h to oviductal fluid. These results suggest that the factor(s) in secretion from the oviduct reduces polyspermic fertilization and the number of spermatozoa that will penetrate porcine oocytes. The reduction of polyspermic penetration by oviductal secretions may be due to a reduced number of spermatozoa in the fertilization me-dium into an intact acrosome.
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